Extraction and Characterization of Hura crepitans Seed Oil


  • Stella Ulakom Department of Physics and Astronomy, University of Nigeria, Nsukka Enugu State, Nigeria
  • Ezekoye Veronica Department of Physics and Astronomy, University of Nigeria, Nsukka Enugu State, Nigeria
  • Anthony Ofomatah National Centre for Energy, Research and Development
  • David Ezekoye Department of Civil Engineering, University of Nigeria, Nsukka, Nigeria
  • Ada Agbogu Department of Physics and Astronomy, University of Nigeria, Nsukka Enugu State, Nigeria




Hura crepitans, seed oil, biodiesel, physicochemical properties, transesterification


Hura crepitans (HC) seed oil was extracted using soxhlet extraction method with n-hexane as solvent at 66.0℃. The extracted oil was converted into biodiesel using alkali-catalytic-transesterification method at a constant temperature of 60℃ for 60 minutes. The oil yield was (42.00%) while biodiesel yield was (71.86%). The physiochemical properties of the analyzed biodiesel were specific gravity (0.902), density (903 kg/m3) at 30℃, viscosity (2.4 mm2/s) at 40℃, flash point (193.0℃), pour point (-10.0℃), cloud point (-2.0℃), acid value (Nil), iodine value (82.0 %), ash content (0.03%) moisture content (0.04%), calorific value (34,860 KJ/kg), Sulphur content (0.01%) Boiling point (202℃), calcium Ca (32.6667 mg/kg), Magnesium Mg (4.42 mg/kg), potassium K (2.32 mg/kg) and Sodium Na (2.15 mg/kg) and phosphorus (0.20, 0.10 mg/kg). Gas chromatography was used to determine biodiesel compositions. Infrared spectroscopy was used for the detection of functional groups and identification of organic compounds. The quality parameters of the biodiesel were found to be within international acceptable ASTM 6751 and EN14214 standards. The Hura crepitans (HC) is a good feedstock for biodiesel production.


Y. G. Keneni and J. M. Marchetti, “Oil extraction from plant seeds for biodiesel production,” vol. 5, no. March, pp. 316–340, 2017, doi: 10.3934/energy.2017.2.316.

A. Ef, E. Betiku, I. Dio, and O. Tv, “Production of biodiesel from crude neem oil feedstock and its emissions from internal combustion engines,” vol. 11, no. 22, pp. 6178–6186, 2012, doi: 10.5897/AJB11.2301.

H. Mulimani, M. C. Navindgi, and T. P. Engineering, “Extraction of biodiesel from vegetable,” vol. 2, pp. 242–250, 2012.

V. Senthilkumar, P. Gunasekaran. Bioethanol production from cellulosic substrate: Engineering bacteria and process integration vol. 64, pp.845-853, 2005.

A. Ramadhas, S. Jayaraj, C. Muraleedharan. Theoretical modelling and experimental studies on biodiesel fueled engine, vol. 31, pp. 1813-1826

B. Metz and C. I. W. G. Iii, “Mitigation of Climate Change Fourth Assessment Report,” 2007.

Nabi, M.N., Rahman, M.M. and Akhter, M.S. (2009) Biodiesel from Cotton Seed Oil and Its Effect on Engine Performance and Exhaust Emissions. Applied Thermal Engineering, 29, 2265-2270. https://doi.org/10.1016/j.applthermaleng.2008.11.009

P. K. Sharma et al., “rip t N ot Co py ed ite d Ac ce pt ed t N ot Co py ed d,” no. c, 2019, doi: 10.1115/1.4042717.

No SY (2011) Inedible vegetable oils and their derivatives for alternative diesel fuels in CI engines: a review. Renew Sust Energ Rev 15: 131–149

M. Gumus and S. Kasifoglu, “Performance and emission evaluation of a compression ignition engine using a biodiesel (apricot seed kernel oil methyl ester) and its blends with diesel fuel,” Biomass and Bioenergy, vol. 34, no. 1, pp. 134–139, 2010, doi: 10.1016/j.biombioe.2009.10.010.

A. K. Azad, M. G. Rasul, M. M. K. Khan, S. C. Sharma, and M. A. Hazrat, “Prospect of biofuels as an alternative transport fuel in Australia,” Renew. Sustain. Energy Rev., vol. 43, pp. 331–351, 2015, doi: 10.1016/j.rser.2014.11.047.

D. Singh, D. Sharma, S. L. Soni, S. Sharma, and P. K. Sharma, “Review article A review on feedstocks , production processes , and yield for di ff erent generations of biodiesel,” Fuel, no. July, p. 116553, 2019, doi: 10.1016/j.fuel.2019.116553.

M. Shahabuddin, A. M. Liaquat, H. H. Masjuki, M. A. Kalam, and M. Mofijur, “Ignition delay , combustion and emission characteristics of diesel engine fueled with biodiesel,” Renew. Sustain. Energy Rev., vol. 21, pp. 623–632, 2013, doi: 10.1016/j.rser.2013.01.019.

Z. Hu, P. Tan, X. Yan, and D. Lou, “Life cycle energy, environment and economic assessment of soybean-based biodiesel as an alternative automotive fuel in China,” vol. 33, pp. 1654–1658, 2008, doi: 10.1016/j.energy.2008.06.004.

M. J. Abedin, H. H. Masjuki, M. A. Kalam, A. Sanjid, S. M. A. Rahman, and I. M. R. Fattah, “Performance , emissions , and heat losses of palm and jatropha biodiesel blends in a diesel engine,” Ind. Crop. Prod., vol. 59, pp. 96–104, 2014, doi: 10.1016/j.indcrop.2014.05.001.

W. Shi et al., “Bioresource Technology Biodiesel production from waste chicken fat with low free fatty acids by an integrated catalytic process of composite membrane and sodium methoxide,” Bioresour. Technol., vol. 139, pp. 316–322, 2013, doi: 10.1016/j.biortech.2013.04.040.

T. Nguyen, L. Do, and D. A. Sabatini, “Biodiesel production via peanut oil extraction using diesel-based reverse-micellar microemulsions,” Fuel, vol. 89, no. 9, pp. 2285–2291, 2010, doi: 10.1016/j.fuel.2010.03.021.

G. Baskar and R. Aiswarya, “Trends in catalytic production of biodiesel from various feedstocks,” Renew. Sustain. Energy Rev., vol. 57, pp. 496–504, 2016, doi: 10.1016/j.rser.2015.12.101.

Y. Tang, X. Gu, and G. Chen, “99 % yield biodiesel production from rapeseed oil using benzyl bromide – CaO catalyst,” pp. 203–208, 2013, doi: 10.1007/s10311-013-0403-9.

M. Kaur and A. Ali, “Lithium ion impregnated calcium oxide as nano catalyst for the biodiesel production from karanja and jatropha oils,” Renew. Energy, vol. 36, no. 11, pp. 2866–2871, 2011, doi: 10.1016. j.renene.2011.04.014.

D. Rajagopal et al., “Linked references are available on JSTOR for this article : Recent Developments in Renewable Technologies : R & D Investment in Advanced Biofuels,” vol. 1, no. 2009, pp. 621–644, 2019.

Demirbaş, A. (2008). Environmental Effects Production of Biodiesel from Algae Oils Production of Biodiesel from Algae Oils. 7036. https://doi.org/10.1080/15567030701521775

Singh, D., Sharma, D., Soni, S. L., Sharma, S., & Sharma, P. K. (2019). Review article A review on feedstocks , production processes , and yield for di ff erent generations of biodiesel. Fuel, July, 116553. https://doi.org/10.1016/j.fuel.2019.116553

Parawira, W. (2010) Biodiesel production from Jatrophacurcas: A review. Scientific Research and Essays, 5(14), 1796-1808.

Highina, B.K., Bugaje, I.M., Umar, B. (2012) Biodiesel Production from Jatropha Caucus Oil in a Batch Reactor Using Zinc Oxide as Catalyst, Journal of Applied Phytotechnology in Environmental Sanitation, 1(2), 61-66.

M. A. Fowomola and A. A. Akindahunsi, Nutrients andAntinutrients of Hura crepitan seed. Journal of Medicinal Food, 10(1):159-164, 2007

M. A. Fowomola and A. A. Akindahunsi, Nutrients andAntinutrients of Hura crepitan seed. Journal of Medicinal Food, 10(1):159-164, 2007

Anthony Cemaluk C. Egbuonu, Olorunshola D. Omodamiro Christian E. Odo1 and Roberts I. Uroko1. Some Antinutritive and Antioxidative Properties of Pulverized Citrus sinensis (Sweet Orange) Peels and Seeds. Journal of Scientific Research Reports,10 (6);1-9

Ibrahim I. A. Yusuf A. J. Extraction and physicochemical analysis of Citrus sinnesis seed oil (sweet orange). European Journal of Experimental Biology, 2015, 5(7): 77-81

Narasimharao K, Lee A, Wilson K. Catalysts in production of biodiesel: a review. Journal of Biobased Materials and Bioenergy 2007; 1: 19–30

Reuther W. The Citrus Industry: Crop protection, postharvest technology, and early history of citrus research in California 3326 UCANR Publications;1967

Hoekman SK, Broch A, Robbins C, Ceniceros E, Natarajan M. Review of biodiesel composition, properties, and specifications. Renewable and Sustainable Energy Reviews 2012; 16: 143–169.

Atabani AE, Silitonga AS, Badruddin IA, Mahlia TMI, Masjuki HH, Mekhilef SA comprehensive review on biodiesel as an alternative energy resource and its characteristics. Renewable and Sustainable Energy Reviews 2012; 16: 2070–2093.

Ezekoye Veronica , Adinde Rita , Ezekoye David , Offormata Anthony , syntheses and characterization of biodiesel from citrus sinensis seed oil, Scientific African (2019), doi: https://doi.org/10.1016/j.sciaf.2019.e00217

Ashraful AM, Masjuki HH, Kalam MA, Fattah IR, Imtenan S, Shahir SA, Mobarak HM. Production and comparison of fuel properties, engine performance, and emission characteristics of biodiesel from various non-edible vegetable oils: a review. Energy Conversion and Management 2014;80: 202–228.

J. O. Oyelade, D. O. Idowu, O. O. Oniya & O. Ogunkunle (2017):Optimization of biodiesel production from sandbox (Hura crepitans L.) seed oil using two different catalysts, Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, doi: 10.1080/15567036.2017.1320691

M. Abdulkadir, I. Amoo, A. Adesina. Investigation of Solvents Efficiencies on Oil Extraction and Biodiesel Production from Hura Crepitans Seeds 2013; 3: 2319 – 7064

Ahmed F, Giwa SO, Ibrahim M, Giwa A. Production of biodiesel from Jatropha curcas seed oil using base catalysed transesterification. International Journal of ChemTech Research 2016; 9: 322–332.

Pramanik K. Properties and use of Jatropha curcas oil and diesel fuel blends in compression ignition engine. Renewable Energy 2003; 28 :239–248.

Ved K, Padam K. Study of physical and chemical properties of biodiesel from sorghum oil. Research Journal of Chemical Sciences 2013; 3: 64–68.

Barabas I, Todorut IA Biodiesel quality, standards and properties. In: Biodiesel-quality, emissions and by. Intech; 2011.

AOAC (Association of Analytical Chemist): Official Method of Analysis 13th ed. (1990). In: William Horwitz. editor, Washington DC; Association of Official Analytical Chemists; 1990 (7) p. 56-132.

Achten WM J, Verchot L, Franken Y, Mathijs E, Singh VP, Aerts R, Muys B. Jatropha bio-diesel production and use. Biomass and Bioenergy, 2008; 32:1063–1084.

Yadav L DS. Organic spectroscopy. Springer Science & Business Media; 2015

Samuel Kofi Tulashie & Francis Kotoka (2019): Kinetics and thermodynamic studies on Moringa oleifera oil extraction for biodiesel production via transesterification, Biofuels, DOI: 10.1080/17597269.2019.169704




How to Cite

Ulakom, S., Ezekoye, V., Ofomatah, A., Ezekoye, D., & Agbogu, A. (2022). Extraction and Characterization of Hura crepitans Seed Oil. Journal of Nano and Materials Science Research, 1, 1–8. https://doi.org/10.20221/jnmsr.v1i.2





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